鸡肌肉组织实时荧光定量PCR及Western杂交内参基因的筛选

doi:10.11843/j.issn.0366-6964.2015.07.005

Abstract

Abstract:

This research evaluated the availability of 5 “housekeeping genes”as reference genes for gene expression detection in different early developmental stages of muscle tissues in chicken，which would provide scientific evidences for selecting reference genes in the molecules detection related to muscle development of chicken.Real-Time quantitative PCR (RT-qPCR) and Western blot were used to test the mRNA and protein levels of β-actin (ACTB)，β-tubulin (TUBB)，TATA-binding-protein (TBP)，Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Heat shock 70 kDa protein (HSP70) in breast muscle of chicken at embryonic day 12 and 17，day 1 and day 14 post-hatch，respectively.The results showed that，only mRNA and protein levels of HSP70 didn’t significantly change in the early developmental stages of breast muscle in chicken，while that of other genes significantly altered (P<0.05 or P<0.01).HSP70 was more suitably used as reference gene for gene or protein expression detection in the early developmental stages of muscle tissues in chicken.This study provides methodological basis for functional genes and proteins studies of muscle development in chicken.It also could play a referential role in other species.

CLC Number:

S831.2

WANG Hong-yang，LIU Ran-ran，ZHAO Gui-ping，ZHENG Mai-qing，LI Qing-he，LI Peng，LIU Li，WEN Jie. Identification of Reference Genes by Real-Time Quantitative PCR and Western Blot in Chicken Muscle Tissues[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1107-1113.

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Identification of Reference Genes by Real-Time Quantitative PCR and Western Blot in Chicken Muscle Tissues

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